{"id":1011,"date":"2013-04-18T11:05:32","date_gmt":"2013-04-18T03:05:32","guid":{"rendered":"http:\/\/www.hzaumycology.com\/chenlianfu_blog\/?p=1011"},"modified":"2013-04-18T12:32:24","modified_gmt":"2013-04-18T04:32:24","slug":"%e5%9f%ba%e5%9b%a0%e7%bb%84%e7%bb%84%e8%a3%85%e8%bd%af%e4%bb%b6%e5%bf%83%e5%be%97","status":"publish","type":"post","link":"http:\/\/www.chenlianfu.com\/?p=1011","title":{"rendered":"\u57fa\u56e0\u7ec4\u7ec4\u88c5\u8f6f\u4ef6\u5fc3\u5f97"},"content":{"rendered":"<h2>1. SOAPdenovo \u548c Velvet\u7ec4\u88c5\u57fa\u56e0\u7ec4\u662f\u5426\u9700\u8981\u5bf9reads\u8fdb\u884c\u9884\u5904\u7406\uff1f<\/h2>\n<p>\u4f7f\u7528soapdenovo\u7ec4\u88c5\u57fa\u56e0\u7ec4\uff0c\u9700\u8981\u5148\u5bf9reads\u8fdb\u884c\u9884\u5904\u7406\uff0c\u53bb\u6389\u8d28\u91cf\u4e0d\u597d\u7684reads\uff0c\u526a\u63893&#8217;\u7aef\u548c5&#8217;\u7aef\u7684\u4e00\u4e9b\u78b1\u57fa\uff1b\u7136\u540e\u518d\u8fdb\u884c\u7ec4\u88c5\u3002\u800c\u4f7f\u7528velvet\u65f6\u5019\uff0c\u5219\u4e0d\u9700\u8981\uff0c\u53ef\u80fd\u662f\u7531\u4e8evelvet\u7684cutoff\u6bd4\u8f83\u597d\u3002<br \/>\n\u8fd9\u4e2a\u7ed3\u8bba\u5f97\u51fa\u65b9\u6cd5\uff1a\u5c06reads\u8fdb\u884c\u9884\u5904\u7406\u524d\u540e\u5206\u522b\u7528\u4e8e\u7ec4\u88c5\u51fa\u57fa\u56e0\u7ec4\uff0c\u7136\u540e\u518d\u4f7f\u7528bowtie2\u5c06\u4e24\u79cdreads\u6bd4\u5bf9\u5230\u4e24\u79cd\u57fa\u56e0\u7ec4\u7ec4\u88c5\u7ed3\u679c\uff0c\u89c2\u5bdfreads\u6bd4\u5bf9\u4e0a\u7684\u767e\u5206\u6bd4\u5f97\u51fa\u3002reads\u7684\u9884\u5904\u7406\u65b9\u6cd5\u662f\u53bb\u9664\u4f4e\u8d28\u91cfreads\u5e76\u5c06100bp\u7684reads\u524d\u540e\u5404\u51cf\u53bb5bp\u78b1\u57fa\u3002<br \/>\n\u5bf9\u4e8esoapdenovo:<\/p>\n<pre>\r\n                      \u672a\u5904\u7406reads\u6bd4\u5bf9\u7387         \u9884\u5904\u7406reads\u6bd4\u5bf9\u7387\r\n\u672a\u5904\u7406reads\u57fa\u56e0\u7ec4          65.84%                   66.10%\r\n\u9884\u5904\u7406reads\u57fa\u56e0\u7ec4          66.68%                   66.99%\r\n<\/pre>\n<p>\u5bf9\u4e8evelvet:<\/p>\n<pre>\r\n                      \u672a\u5904\u7406reads\u6bd4\u5bf9\u7387         \u9884\u5904\u7406reads\u6bd4\u5bf9\u7387\r\n\u672a\u5904\u7406reads\u57fa\u56e0\u7ec4          86.41%                   88.10%\r\n\u9884\u5904\u7406reads\u57fa\u56e0\u7ec4          70.09%                   71.62%\r\n<\/pre>\n<p>\u901a\u8fc7\u4ee5\u4e0a\u7684\u5bf9\u6bd4\u548c\u6570\u636e\u53ef\u4ee5\u770b\u51fa\uff1a1.velvet\u7ec4\u88c5\u51fa\u7684\u57fa\u56e0\u7ec4\u7684\u7cbe\u786e\u6027\u8981\u6bd4soapdenovo\u9ad8\uff1b2.\u5bf9reads\u8fdb\u884c\u9884\u5904\u7406\u5bf9soapdenovo\u7ec4\u88c5\u7684\u57fa\u56e0\u7ec4\u7684\u7cbe\u786e\u6027\u5f71\u54cd\u8f83\u5c0f\uff0c\u9884\u5904\u7406reads\u540e\uff0c\u7ec4\u88c5\u51fa\u7684\u57fa\u56e0\u7ec4\u7cbe\u786e\u6027\u7a0d\u6709\u63d0\u9ad8\uff1b3.\u4f7f\u7528velvet\u7ec4\u88c5\u57fa\u56e0\u7ec4\u65f6\uff0c\u4e0d\u5bf9reads\u8fdb\u884c\u9884\u5904\u7406\u7ec4\u88c5\u51fa\u7684\u57fa\u56e0\u7ec4\u7cbe\u786e\u6027\u9ad8\u5f88\u591a\u3002<br \/>\n\u8ba8\u8bba\uff1a1.\u6570\u636e\u91cf\u4e0d\u591f\u591a\uff0c\u78b1\u57fa\u8986\u76d6\u5ea6\u53ea\u670920~25X, \u4ee5\u4e0a\u7ed3\u8bba\u5177\u6709\u4e00\u5b9a\u7684\u7247\u9762\u6027; 2.velvet\u5728\u5bf9kmer\u8fdb\u884ccutoff\u7684\u7b97\u6cd5\u5f88\u68d2\uff0c\u56e0\u800c\u7ec4\u88c5\u51fa\u7684\u57fa\u56e0\u7ec4\u7cbe\u786e\u6027\u9ad8\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>1. SOAPdenovo \u548c Velvet\u7ec4\u88c5\u57fa\u56e0\u7ec4\u662f\u5426\u9700\u8981\u5bf9reads\u8fdb\u884c\u9884 &hellip; <a href=\"http:\/\/www.chenlianfu.com\/?p=1011\">\u7ee7\u7eed\u9605\u8bfb <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[39],"_links":{"self":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/1011"}],"collection":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1011"}],"version-history":[{"count":7,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/1011\/revisions"}],"predecessor-version":[{"id":2427,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/1011\/revisions\/2427"}],"wp:attachment":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1011"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1011"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1011"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}