{"id":111,"date":"2012-08-22T21:20:44","date_gmt":"2012-08-22T13:20:44","guid":{"rendered":"http:\/\/www.hzaumycology.com\/chenlianfu_blog\/?p=111"},"modified":"2012-08-22T22:09:14","modified_gmt":"2012-08-22T14:09:14","slug":"blast%e6%9c%ac%e5%9c%b0%e5%8c%96web%e8%bf%90%e7%94%a8","status":"publish","type":"post","link":"http:\/\/www.chenlianfu.com\/?p=111","title":{"rendered":"BLAST\u672c\u5730\u5316web\u8fd0\u7528"},"content":{"rendered":"

1 \u9996\u5148\uff0c\u5230ftp:\/\/ftp.ncbi.nih.gov\/blast\/executables\/release\/LATEST\/\u4e0b\u8f7d\u6700\u65b0\u7684\u7248\u672c\u7684wwwblast\uff08\u5199\u6b64\u6587\u65f6\u5bf9\u5e94\u7684\u6587\u4ef6\u4e3awwwblast-2.2.26-x64-linux.tar.gz\uff09\u3002<\/h1>\n

2 \u7136\u540e\u5c06\u8be5\u6587\u4ef6\u89e3\u538b\uff0c\u4e0d\u9700\u8981configure\u5373\u53ef\u4f7f\u7528\u3002\u8bbe\u5b9a\u6240\u89e3\u538b\u7684\u6587\u4ef6\u5939\u5230Apache\u8def\u5f84\u3002<\/h1>\n

\u5c06\u5982\u4e0b\u51e0\u884c\u52a0\u5165\/etc\/httpd\/conf\/httpd.conf\u4e2d\uff1a<\/p>\n

Alias \/blast \"\/home\/chenlianfu\/programs\/wwwblast\"\r\n<Directory \"\/home\/chenlianfu\/programs\/wwwblast\">\r\n\u00a0\u00a0\u00a0 Options ExecCGI\r\n\u00a0\u00a0\u00a0 AllowOverride None\r\n\u00a0\u00a0\u00a0 Order allow,deny\r\n\u00a0\u00a0\u00a0 Allow from all\r\n<\/Directory><\/pre>\n
3 wwwblast\u9ed8\u8ba4\u63d0\u4f9b\u4e862\u4e2atest\u7684\u6570\u636e\u5e93\uff0c\u5206\u522b\u4e3atest_na_db\u548ctest_aa_db\u3002<\/h1>\n
4 \u4f7f\u7528\u6b63\u5e38\u7248\u7684 makeblastdb \u6765\u521b\u5efa\u6570\u636e\u5e93\uff0c\u5e76\u5c06\u6570\u636e\u5e93\u6587\u4ef6\u653e\u5165\u6587\u4ef6\u5939\u7684db\/\u8def\u5f84\u4e0b\u3002<\/h1>\n
5. \u4fee\u6539 blast.html \u6587\u4ef6\u3002<\/h1>\n
<select name = \"DATALIB\">\r\n    <option VALUE = \u201cnt\"> nt #nt\u662fdb\/\u4e2d\u6240\u542b\u6709\u7684\u6570\u636e\u5e93\u540d\u3002\r\n<\/select><\/pre>\n
6. \u4fee\u6539 blast.rc \u914d\u7f6e\u6587\u4ef6\u3002<\/h1>\n
NumCpuToUse     6\t\uff03CPU\u7684\u4f7f\u7528\u4e2a\u6570\uff0c\u7ebf\u7a0b\u6570\u3002\r\n\r\nblastn nt\t\uff03blastn\u7a0b\u5e8f\u6240\u80fd\u4f7f\u7528\u7684\u6570\u636e\u5e93\u540d\u79f0\u3002\r\ntblastn nt\t\uff03\u4e0b\u540c...\r\ntblastx nt \r\nblastp \r\nblastx<\/pre>\n
5 \u8fd9\u6837\uff0c\u5728\u7f51\u9875\u4e2d\u5c31\u80fd\u6b63\u5e38\u4f7f\u7528nt\u6570\u636e\u5e93\u4e86\u3002<\/h1>\n","protected":false},"excerpt":{"rendered":"
1 \u9996\u5148\uff0c\u5230ftp:\/\/ftp.ncbi.nih.gov\/blast\/execu … \u7ee7\u7eed\u9605\u8bfb →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[8],"_links":{"self":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/111"}],"collection":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=111"}],"version-history":[{"count":5,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/111\/revisions"}],"predecessor-version":[{"id":132,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/111\/revisions\/132"}],"wp:attachment":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=111"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=111"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=111"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}