{"id":1795,"date":"2013-08-27T14:00:58","date_gmt":"2013-08-27T06:00:58","guid":{"rendered":"http:\/\/www.hzaumycology.com\/chenlianfu_blog\/?p=1795"},"modified":"2013-10-12T11:42:05","modified_gmt":"2013-10-12T03:42:05","slug":"%e6%88%91%e7%9a%84%e7%ac%ac%e4%b8%80%e5%b1%8a%e7%94%9f%e7%89%a9%e4%bf%a1%e6%81%af%e5%ad%a6%e5%9f%b9%e8%ae%ad%e7%8f%ad%e6%80%bb%e7%bb%93","status":"publish","type":"post","link":"http:\/\/www.chenlianfu.com\/?p=1795","title":{"rendered":"\u6211\u7684\u7b2c\u4e00\u5c4a\u751f\u7269\u4fe1\u606f\u5b66\u57f9\u8bad\u73ed\u603b\u7ed3"},"content":{"rendered":"

1 \u57f9\u8bad\u7684\u51c6\u5907<\/h1>\n

\u5728\u4eca\u5e744\u6708\u4efd\u7684\u65f6\u5019\u5c31\u840c\u751f\u4e86\u4e00\u4e2a\u60f3\u6cd5\uff0c\u60f3\u5c06\u81ea\u5df1\u5b66\u5230\u7684\u4e00\u4e9b\u751f\u7269\u4fe1\u606f\u5b66\u77e5\u8bc6\u5206\u4eab\u7ed9\u5b83\u4eba\uff0c\u540c\u65f6\u4e5f\u8d5a\u70b9\u751f\u6d3b\u8d39\u3002\u540e\u6765\u5076\u7136\u5728\u4e00\u4e2a\u751f\u7269\u4fe1\u606f\u7684\u7fa4\u91cc\u9762\uff0c\u6709\u4eba\u8bf4\u5f88\u60f3\u5728\u6691\u671f\u53bb\u4e0a\u751f\u7269\u4fe1\u606f\u5b66\u73ed\uff0c\u5b66\u4e60\u5230\u5b9e\u7528\u7684\u77e5\u8bc6\uff1b\u540c\u65f6\u53c8\u62b1\u6028\u534e\u5927\u7b49\u4e3e\u529e\u7684\u751f\u7269\u4fe1\u606f\u5b66\u57f9\u8bad\u73ed\u4e0d\u4ec5\u5b66\u8d39\u8d35\uff0c\u540c\u65f6\u4e5f\u5b66\u4e0d\u5230\u81ea\u5df1\u52a8\u624b\u7684\u4e1c\u897f\u3002\u4e8e\u662f\u6211\u5c31\u5199\u5230\u8003\u8651\u5728\u6691\u671f\u529e\u4e00\u4e2a\u57f9\u8bad\u73ed\u3002\u4e8e\u662f\u5f53\u65f6\u4e00\u51b2\u52a8\uff0c\u5c31\u5728\u534e\u4e2d\u519c\u4e1a\u5927\u5b66\u7684\u5357\u6e56\u8bba\u575b\u4e0a\u53d1\u4e86\u4e00\u4e2a\u5e16\u5b50\uff0c\u8bf4\u60f3\u8981\u5f00\u4e00\u4e2a\u751f\u7269\u4fe1\u606f\u5b66\u7684\u73ed\u3002\u540e\u6765\u5f88\u591a\u4eba\u56de\u590d\u5e76\u611f\u5174\u8da3\uff0c\u4e8e\u662f\u8fd9\u6210\u4e86\u6211\u4e3e\u529e\u751f\u7269\u4fe1\u606f\u5b66\u57f9\u8bad\u73ed\u7684\u6700\u5927\u52a8\u529b\u3002\u540e\u6765\u5728\u4e00\u4e9b\u56de\u590d\u7684\u8981\u6c42\u4e0b\u5efa\u7acb\u4e86\u4e00\u4e2aqq\u7fa4\uff0c\u4e8e\u662f\u5c06\u57f9\u8bad\u73ed\u7684\u4e8b\u4e1a\u62c9\u5012\u6b63\u8f68\u4e0a\u3002<\/p>\n

\u5f00\u73ed\u57f9\u8bad\u73ed\uff0c\u5219\u5fc5\u987b\u8981\u6709\u57f9\u8bad\u8d44\u6599\uff0c\u4e8e\u662f\u5c31\u5f00\u59cb\u6574\u7406\u57f9\u8bad\u8d44\u6599\u3002\u5176\u5b9e\u8fd9\u4e9b\u57f9\u8bad\u8d44\u6599\u5f88\u591a\u90fd\u662f\u53d6\u81ea\u6211\u96f6\u96f6\u6563\u6563\u5199\u5230\u6211\u7684blog\u4e0a\u7684\u5185\u5bb9\u3002\u5728\u4eca\u5e74\u6691\u50477.14-7.24\u65e5\uff0c\u6211\u53c2\u52a0\u4e86\u590d\u65e6\u5927\u5b66\u4e3e\u529e\u7684\u4e00\u4e2a\u8fdb\u5316\u5206\u6790\u7684\u57f9\u8bad\u73ed\u3002\u90a3\u4e2a\u65f6\u5019\u767d\u5929\u4e0a\u8bfe\uff0c\u665a\u4e0a\u5c31\u5728\u5bbe\u9986\u5199\u57f9\u8bad\u8d44\u6599\uff0c\u540c\u65f6\u51c6\u5907\u4e0a\u8bfe\u7684\u6570\u636e\u3002\u57f9\u8bad\u8d44\u6599\u548c\u6570\u636e\u7684\u51c6\u5907\u7edd\u5927\u90e8\u5206\u662f\u5728\u90a3\u4e2a\u65f6\u5019\u5b8c\u6210\u7684\uff0c\u5f53\u7136\uff0c\u7531\u4e8e\u6bd4\u8f83\u5306\u5fd9\uff0c\u5728\u6700\u540e\u4e00\u7ae0\u7684\u5185\u5bb9\u5c31\u5199\u5f97\u7c97\u7cd9\u4e86\uff0c\u540c\u65f6\u57f9\u8bad\u8d44\u6599\u4e2d\u8fd8\u6709\u5f88\u591a\u7684\u9519\u522b\u5b57\u7b49\u3002<\/p>\n

\u901a\u8fc7qq\u7fa4\u7684\u901a\u77e5\uff0c\u57f9\u8bad\u65f6\u95f4\u786e\u5b9a\u57287.27\u65e5\u4e0b\u53483\u70b9\u8fdb\u884c\u4e00\u4e2a\u8bd5\u8bb2\uff0c\u8bb2\u8ff0\u57f9\u8bad\u7684\u5927\u7eb2\uff1b\u800c\u4ece7.28\u65e5\u5f00\u59cb\u6b63\u5f0f\u7684\u8bb2\u8bfe\u3002<\/p>\n

2 \u57f9\u8bad\u5185\u5bb9<\/h2>\n

\u8fd9\u6b21\u7684\u751f\u7269\u4fe1\u606f\u57f9\u8bad\u4e3b\u8981\u662fNGS\u6570\u636e\u7684\u5206\u6790\uff0c\u662f\u73b0\u5728\u6bd4\u8f83\u6210\u719f\u53c8\u70ed\u95e8\u7684\u77e5\u8bc6\u3002\u8bb2\u89e3\u4e86\u57fa\u56e0\u7ec4\u7684\u7ec4\u88c5\u3001\u57fa\u56e0\u9884\u6d4b\u3001SNP\u5206\u6790\u3001\u8f6c\u5f55\u7ec4\u5206\u6790\u3001\u57fa\u56e0\u529f\u80fd\u6ce8\u91ca\u7b49\uff0c\u8fd8\u5305\u542b\u4e86\u7f51\u7ad9\u642d\u5efa\uff0cmysql\u4f7f\u7528\u548cperl\u7f16\u7a0b\u7b49\u3002\u5168\u7a0b\u8bfe\u7a0b\u4e3b\u8981\u504f\u91cd\u5b9e\u8df5\u8fd0\u7528\u4e8e\u4e0a\u673a\u64cd\u4f5c\uff0c\u800c\u539f\u7406\u65b9\u9762\u5219\u4e0d\u4f1a\u6df1\u7a76\u3002<\/p>\n

\u672c\u6b21\u57f9\u8bad\u7684\u5927\u7eb2\u5982\u4e0b\uff1a<\/p>\n

\r\n1.CentOS 6.4 x86_64\u7684\u5b89\u88c5\r\n2.Linux\u7cfb\u7edf\u5165\u95e8\r\n3.Next Generation Sequencing Techonology\r\n4.\u57fa\u56e0\u7ec4\u7ec4\u88c5\r\n5.Genom Repeat Sequence Prediction\r\n6.\u77ed\u5e8f\u5217\u6bd4\u5bf9\r\n7.Perl\u5165\u95e8\r\n8.Variants calling\r\n9.\u8f6c\u5f55\u7ec4\u5206\u6790\r\n10.\u57fa\u56e0\u9884\u6d4b\r\n11.\u57fa\u56e0\u7ec4\u6d4f\u89c8\u5668Gbrowse\r\n12.\u57fa\u56e0\u529f\u80fd\u6ce8\u91ca\u4e0e\u5bcc\u96c6\u5206\u6790\r\n<\/pre>\n
\u57f9\u8bad\u8bb2\u4e49\u8d44\u6599\u6709151\u9762\uff0c\u5185\u5bb9\u8fd8\u662f\u5f88\u4e30\u5bcc\u7684\u3002<\/p>\n
3 \u57f9\u8bad\u60c5\u51b5<\/h1>\n
\u57287.27\u65e5\u4e0b\u53483\u70b9\u5f00\u59cb\u57f9\u8bad\u524d\u8bd5\u8bb2\uff0c\u5f53\u65f6\u5929\u6c14\u5f88\u70ed\uff0c\u7a7a\u8c03\u521a\u5f00\uff0c\u4f46\u662f\u5927\u51fa\u6211\u7684\u9884\u6599\uff0c\u6765\u4e86\u5f88\u591a\u4eba\uff0c\u5c06\u4f1a\u8bae\u5ba4\u90fd\u6324\u6ee1\u4e86\u3002\u73b0\u573a\u62a5\u540d\uff0c\u62a5\u540d\u8d39\u76f8\u5bf9\u4fbf\u5b9c\uff0c500\u5143\u3002\u7531\u4e8e\u573a\u5730\u4e0d\u591f\u5927\uff0c\u4e8e\u662f\u968f\u673a\u62bd\u53d6\u4e86\u90e8\u5206\u6709\u610f\u5411\u7684\u4eba\u62a5\u540d\uff0c\u6700\u540e\u603b\u5171\u62a5\u540d\u4eba\u657027\u4eba\u3002\u5f53\u7136\uff0c\u6709\u4e00\u4e2a\u9ad8\u7ea7\u535a\u540e\u4ece\u4e0a\u6d77\u4e13\u7a0b\u5230\u8fd9\u513f\u6765\u542c\u8bfe\u7684\uff0c\u50cf\u8fd9\u6837\u7684\u9700\u8981\u4f18\u5148\u8003\u8651\u3002\u62a5\u540d\u65f6\uff0c\u5f53\u65f6\u6536\u94b1\u7684\u611f\u89c9\u8fd8\u662f\u5f88\u723d\u7684\uff0c\u563f\u563f…\u8fd9\u7b97\u662f\u6211\u4eba\u751f\u7684\u7b2c\u4e00\u6876\u91d1\uff0c\u4f46\u662f\u7d27\u63a5\u800c\u6765\u5219\u662f\u8981\u591a\u5b66\u5458\u8d1f\u8d23\uff0c\u8981\u6559\u7ed9\u4ed6\u4eec\u6709\u7528\u7684\u77e5\u8bc6\u4e86\u3002<\/p>\n
\u57f9\u8bad\uff0c\u51c6\u786e\u8bf4\u662f\u96c6\u8bad\uff0c\u4ece7.28\u65e5\u5f00\u59cb\uff0c\u6bcf\u5929\u65e9\u4e0a9:00-12:00\uff0c\u4e0b\u53482:00-5:00\uff0c\u665a\u4e0a7:00-9:00\u4e0a\u8bfe\u3002\u8fd9\u6837\u603b\u5171\u6301\u7eed\u4e8610\u5929\uff0c\u5176\u4e2d\u5305\u542b\u653e\u5047\u4e86\u4e00\u5929\u3002\u521a\u5f00\u59cb\u4e24\u5929\uff0c\u65e9\u4e2d\u665a\u90fd\u8bb2\u8bfe\uff0c\u540e\u6765\u665a\u4e0a\u5c31\u4e13\u95e8\u7528\u6765\u7ec3\u4e60\uff0c\u624b\u628a\u624b\u7b54\u7591\u4e86\u3002\u6700\u540e\u4e00\u5929\uff0c\u4eba\u4eba\u90fd\u5f88\u7d2f\uff0c\u540c\u65f6\u57f9\u8bad\u8d44\u6599\u6700\u540e\u4e00\u7ae0\u4e5f\u5199\u5f97\u6bd4\u8f83\u7b80\u7565\uff0c\u6570\u636e\u4e5f\u51c6\u5907\u7684\u4e0d\u662f\u5f88\u5145\u5206\uff0c\u4e8e\u662f\u7ed3\u675f\u8bfe\u7a0b\u4e5f\u7b97\u6bd4\u8f83\u5306\u5fd9\u3002\u60f3\u8d77\u6765\uff0c\u5f53\u521d\u6211\u505a\u521a\u751f\u7269\u4fe1\u606f\u5b66\uff0c\u5c31\u662f\u4ece\u6700\u540e\u4e00\u7ae0\u57fa\u56e0\u529f\u80fd\u6ce8\u91ca\u7684\u5185\u5bb9\u5f00\u59cb\u7684\uff0c\u5bf9\u8fd9\u4e00\u7ae0\u7684\u5185\u5bb9\uff0c\u5176\u5b9e\u4e86\u89e3\u5f97\u975e\u5e38\u6df1\u5165\u3002<\/p>\n
\u6700\u540e\uff0c\u6240\u6709\u7684\u5b66\u5458\u5728\u5b9e\u9a8c\u5ba4\u95e8\u524d\u5408\u4e86\u4e00\u5f20\u5f71\uff0c\u4f5c\u4e3a\u7559\u604b\uff1a
\n\"\u9648\u8fde\u798f\u7684\u7b2c\u4e00\u5c4a\u57f9\u8bad\u73ed\u5b66\u5458\u5408\u5f711\"
\n\"\u9648\u8fde\u798f\u7684\u7b2c\u4e00\u5c4a\u57f9\u8bad\u73ed\u5b66\u5458\u5408\u5f712\"<\/p>\n
4 \u6211\u7684\u57f9\u8bad\u6536\u83b7<\/h1>\n
\u901a\u8fc7\u8fd9\u6b21\u57f9\u8bad\uff0c\u6211\u8ba4\u8bc6\u4e86\u4e00\u4e9b\u4eba\uff0c\u5f88\u591a\u4eba\u7ed9\u6211\u7559\u4e0b\u4e86\u6df1\u523b\u7684\u5370\u8c61\u3002\u6bd4\u5982\uff1a\u4ece\u4e0a\u6d77\u7279\u5f02\u8fc7\u6765\u7684\u9ad8\u7ea7\u535a\u540e\uff0c\u672c\u8eab\u5bf9NGS\u5206\u6790\u5f88\u611f\u5174\u8da3\uff0c\u5b66\u5f97\u5f88\u5feb\uff1b\u57fa\u56e0\u697c\u7684\u7231\u5b66\u4e60\uff0c\u559c\u6b22\u8fa9\u8bba\u7684\u5c0f\u4f19\u5b50\uff1b\u5728\u6d4b\u5e8f\u516c\u53f8\u5de5\u4f5c\u51e0\u5e74\u540e\u56de\u6765\u7ee7\u7eed\u8bfb\u4e66\u7684\uff0c\u95ee\u9898\u5f88\u591a\u7684\u540c\u5b66\uff1b\u8fd8\u6709\u521a\u53d1\u8868\u4e86Nature techonology\u9a6c\u4e0a\u53bb\u745e\u58eb\u8bfb\u535a\u58eb\u7684\u725b\u4eba\uff1b\u5728\u7a7a\u8c03\u53e3\u5439\u98ce\u53c8\u5f88\u6d3b\u8dc3\u7684\u4e00\u7fa4\u4eba\uff1b\u4e5f\u6709\u559c\u6b22\u94bb\u7814\u7684\u5973\u751f\u7b49\u3002<\/p>\n
\u57f9\u8bad\u8bfe\u4e0a\u4e5f\u6709\u4e00\u4e9b\u540c\u5b66\u9000\u5b66\uff0c\u4e5f\u6709\u4e00\u4e9b\u540c\u5b66\u52a0\u5165\uff0c\u4e5f\u6709\u4eba\u82e6\u82e6\u652f\u6491\u5230\u6700\u540e\u3002<\/p>\n
\u4f5c\u4e3a\u8001\u5e08\uff0c\u6211\u4e5f\u5b66\u5230\u4e86\u5f88\u591a\u4e0a\u8bfe\u7ecf\u9a8c\u3002\u5728\u57f9\u8bad\u8bfe\u4e0a\uff0c\u4e0a\u8bfe\u662f\u8bb2\u4e00\u4f1a\u513f\uff0c\u4e0a\u673a\u4e00\u4f1a\u513f\uff0c\u9020\u6210\u4e86\u8bfe\u95f4\u4f11\u606f\u4e0d\u89c4\u5f8b\uff1b\u4e0a\u8bfe\u8282\u594f\u8981\u6162\uff0c\u5410\u8bcd\u8981\u6e05\u6670\u7b49\u3002\u5f53\u7136\uff0c\u5bf9\u57f9\u8bad\u8bfe\u7a0b\u8fdb\u5ea6\u7684\u628a\u63e1\uff0c\u73b0\u5728\u6709\u4e86\u7ecf\u9a8c\u3002\u539f\u672c\u51c6\u5907\u57283~5\u5929\u5185\u8bb2\u5b8c\uff0c\u4f46\u662f\u5728\u5f88\u5feb\u7684\u8282\u594f\u4e0b\u4e5f\u9700\u8981\u4e869\u5929\u7684\u65f6\u95f4\u3002<\/p>\n
5 \u603b\u7ed3<\/h1>\n
\u672c\u6b21\u57f9\u8bad\uff0c\u901a\u8fc7\u624b\u628a\u624b\u6559\u5bfc\u600e\u4e48\u8fdb\u884cNGS\u5206\u6790\u4e0e\u4e0a\u7ea7\u64cd\u4f5c\uff0c\u5b66\u5458\u4eec\u6700\u540e\u80fd\u72ec\u81ea\u81ea\u4e3b\u8fdb\u884c\u9ad8\u8d28\u91cf\u7684\uff1a\u57fa\u56e0\u7ec4\u7ec4\u88c5\uff0cSNP\u548cINDEL\u68c0\u6d4b\uff0c\u57fa\u56e0\u9884\u6d4b\u53ca\u5176\u53ef\u89c6\u5316\uff0c\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u57fa\u56e0\u529f\u80fd\u6ce8\u91ca\u7b49\u3002<\/p>\n
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