{"id":2406,"date":"2015-12-26T22:40:54","date_gmt":"2015-12-26T14:40:54","guid":{"rendered":"http:\/\/www.chenlianfu.com\/?p=2406"},"modified":"2015-12-26T22:40:54","modified_gmt":"2015-12-26T14:40:54","slug":"blast%e8%bf%9b%e8%a1%8c%e9%87%8d%e5%a4%8d%e5%ba%8f%e5%88%97%e5%b1%8f%e8%94%bd","status":"publish","type":"post","link":"http:\/\/www.chenlianfu.com\/?p=2406","title":{"rendered":"blast\u8fdb\u884c\u91cd\u590d\u5e8f\u5217\u5c4f\u853d"},"content":{"rendered":"

1. \u6784\u5efa\u6570\u636e\u5e93\u7684\u65f6\u5019\u5c4f\u853d\u53c2\u8003\u5e8f\u5217\u7684\u91cd\u590d<\/h1>\n

segmasker \u5c4f\u853d\u6c28\u57fa\u9178\u7684\u4f4e\u590d\u6742\u5e8f\u5217
\ndustmasker \u5c4f\u853d\u6838\u7b97\u5e8f\u5217\u7684\u4f4e\u590d\u6742\u5e8f\u5217
\nwindowmasker \u6309\u7167\u5e8f\u5217\u91cd\u590d\u7684\u6b21\u6570\u6765\u5c4f\u853d
\nconvert2blastmask \u6839\u636e\u5c0f\u5199\u5b57\u6bcd\u6765\u5c4f\u853d<\/p>\n

\u8fd9\u51e0\u4e2a\u90fd\u53ef\u4ee5\u5148\u5f97\u5230\u4e00\u4e2a\u542b\u6709\u5c4f\u853d\u4fe1\u606f\u7684\u6587\u4ef6\u3002\u7136\u540e\u8fdb\u884c makeblastdb \u7684\u65f6\u5019\u8f93\u5165\u8fd9\u4e2a\u6587\u4ef6\uff0c\u5c31\u53ef\u4ee5\u76f8\u5e94\u7684 masked \u6570\u636e\u5e93\u4e86\u3002<\/p>\n

\u53c2\u8003\uff1ahttp:\/\/www.ncbi.nlm.nih.gov\/books\/NBK279681\/<\/a><\/p>\n

2. \u6bd4\u5bf9\u7684\u65f6\u5019\u5bf9query\u5e8f\u5217\u7684\u91cd\u590d\u8fdb\u884c\u5c4f\u853d<\/h1>\n

blast \u6bd4\u5bf9\u7684\u65f6\u5019\uff0c\u53ef\u4ee5\u5bf9 query \u5e8f\u5217\u8fdb\u884c\u5c4f\u853d\u3002 \u8fd9\u51e0\u4e2a\u53c2\u6570\u4f30\u8ba1\u8fd9\u6837\u7406\u89e3\uff1a
\n-seg blastp\u7684\u53c2\u6570\uff0c\u662f\u5426\u5bf9query \u5e8f\u5217\u4f7f\u7528 segmasker\u6765\u5c4f\u853d\u4f4e\u590d\u6742\u91cd\u590d\uff0c\u9ed8\u8ba4 no
\n-dust blastn\u7684\u53c2\u6570\uff0c\u662f\u5426\u5bf9query \u5e8f\u5217\u4f7f\u7528 dustmasker\u6765\u5c4f\u853d\u4f4e\u590d\u6742\u91cd\u590d\uff0c\u9ed8\u8ba4 no
\n-lcase_masking \u5bf9query\u5e8f\u5217\u7684\u5c0f\u5199\u90e8\u5206\u8fdb\u884c\u5c4f\u853d
\n-soft_masking \u662f\u5426\u8fdb\u884c\u8f6f\u5c4f\u853d\u3002\u8f6f\u5c4f\u853d\u5219\u662f\u4e0d\u4f1a\u4f7f\u7528\u5c4f\u853d\u7684\u5e8f\u5217\u8fdb\u884c\u79cd\u5b50\u6bd4\u5bf9\uff0c\u4f46\u662f\u53ef\u4ee5\u5ef6\u957f\u65f6\u5019\u6bd4\u5bf9\u3002\u786c\u5c4f\u853d\uff0c\u5219\u662f\u76f4\u63a5\u4e0d\u5bf9\u5c4f\u853d\u5e8f\u5217\u90e8\u5206\u8fdb\u884c\u6bd4\u5bf9\u3002blastn\u7684\u9ed8\u8ba4\u503c\u662fyes\uff0cblastp\u7684\u9ed8\u8ba4\u503c\u662fno<\/p>\n","protected":false},"excerpt":{"rendered":"

1. \u6784\u5efa\u6570\u636e\u5e93\u7684\u65f6\u5019\u5c4f\u853d\u53c2\u8003\u5e8f\u5217\u7684\u91cd\u590d segmasker \u5c4f\u853d\u6c28\u57fa\u9178\u7684\u4f4e\u590d\u6742 … \u7ee7\u7eed\u9605\u8bfb →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/2406"}],"collection":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2406"}],"version-history":[{"count":1,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/2406\/revisions"}],"predecessor-version":[{"id":2407,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/2406\/revisions\/2407"}],"wp:attachment":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2406"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2406"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2406"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}