{"id":255,"date":"2012-09-17T10:40:53","date_gmt":"2012-09-17T02:40:53","guid":{"rendered":"http:\/\/www.hzaumycology.com\/chenlianfu_blog\/?p=255"},"modified":"2013-10-12T15:45:21","modified_gmt":"2013-10-12T07:45:21","slug":"%e4%bd%bf%e7%94%a8misa%e7%bb%93%e5%90%88primer3%e6%9d%a5%e6%89%b9%e9%87%8f%e8%ae%be%e8%ae%a1ssr%e5%bc%95%e7%89%a9-2","status":"publish","type":"post","link":"http:\/\/www.chenlianfu.com\/?p=255","title":{"rendered":"\u4f7f\u7528Misa\u7ed3\u5408Primer3\u6765\u6279\u91cf\u8bbe\u8ba1SSR\u5f15\u7269"},"content":{"rendered":"

MISA\uff0c\u82f1\u6587\u5168\u79f0\u4e3aMI<\/span>croSA<\/span>tellite identification tool\uff0c\u5373\u5fae\u536b\u661f\u8bc6\u522b\u5de5\u5177\u3002<\/h1>\n

MISA\u662f\u4f7f\u7528 perl \u7f16\u5199\u7684\u4e00\u652f\u7a0b\u5e8f\uff0c\u80fd\u8bc6\u522b\u51fa\u5e8f\u5217\u4e2d\u7684\u5fae\u536b\u661f\u548c\u590d\u5408\u5fae\u536b\u661f\uff08\u4e24\u4e2a\u5fae\u536b\u661f\u4e4b\u95f4\u7531\u7531\u4e0d\u591a\u4e8e100bp\u7684\u78b1\u57fa\u5bf9\u9694\u5f00\uff09\uff0c\u5e76\u7ed9\u51fa\u5176\u6240\u5728\u4f4d\u70b9\u3002<\/p>\n

MISA\u4e0b\u8f7d\u7f51\u5740\uff1ahttp:\/\/pgrc.ipk-gatersleben.de\/misa\/misa.html<\/a><\/p>\n

MISA\u7528\u6cd5\uff1a<\/h1>\n
$ misa.pl filename\r\nmisa.pl <FASTAfile>\r\n\u5176\u4e2d\uff0cfastfile\u662f\u5e8f\u5217\u6587\u4ef6\uff0c\u540c\u65f6\u5728\u8fd0\u884c\u7a0b\u5e8f\u7684\u5de5\u4f5c\u76ee\u5f55\u4e0b\u5fc5\u987b\u6709\u4e00\u4e2a\u540d\u79f0\u4e3a\u201cmisa.ini\u201d\r\n\u7684\u6587\u4ef6\u3002\u8be5\u6587\u4ef6\u5185\u5bb9\u4e3a\uff1a\r\ndefinition(unit_size,min_repeats): 1-10 2-6 3-5 4-5 5-5 6-5\r\ninterruptions(max_difference_for_2_SSRs): 100 \r\n\u8be5\u6587\u4ef6\u6307\u5b9a\u4e86misa\u7684\u53c2\u6570\uff0c\u53731\u4e2a\u78b1\u57fa\u91cd\u590d10\u6b21\u53ca10\u6b21\u4ee5\u4e0a\uff1b2\u4e2a\u78b1\u57fa\u91cd\u590d6\u6b21\u53ca6 \u6b21\u4ee5\u4e0a\uff1b\r\n3\u4e2a\u78b1\u57fa\u91cd\u590d5\u6b21\u53ca5\u6b21\u4ee5\u4e0a\uff1b4\u4e2a\u78b1\u57fa\u91cd\u590d5\u6b21\u53ca5\u6b21\u4ee5\u4e0a\uff1b5\u4e2a\u78b1\u57fa\u91cd\u590d5 \u6b21\u53ca5\u6b21\u4ee5\u4e0a\uff1b6\u78b1\r\n\u57fa\u91cd\u590d5\u6b21\u53ca5\u6b21\u4ee5\u4e0a\uff0c\u8fd9\u6837\u7684\u78b1\u57fa\u91cd\u590d\u5e8f\u5217\u624d\u7b97\u662f\u5fae\u536b\u661f\u5e8f\u5217\u3002 \u540c\u65f6\uff0c\u4e24\u4e2a\u5fae\u536b\u661f\u4e4b\u95f4\u7684\u8ddd\r\n\u79bb\u5c0f\u4e8e100bp\u7684\u65f6\u5019\uff0c\u4e24\u4e2a\u5fae\u536b\u661f\u7ec4\u6210\u4e00\u4e2a\u590d\u5408\u5fae\u536b\u661f\u3002<\/pre>\n
MISA\u7684\u8f93\u51fa\u7ed3\u679c\uff1a<\/h1>\n
MISA\u4f1a\u5728 Fastafile \u6240\u5728\u7684\u6587\u4ef6\u5939\u4e0b\u751f\u6210\u4e24\u4e2a\u6587\u4ef6\uff0c\u5206\u522b\u662f \u201c<FASTfile>.misa\u201d \u548c \u201c<FASTfile>.statistics\u201d<\/p>\n
\"<FASTfile>.misa\" \uff1a\u4ee5\u8868\u683c\u7684\u5f62\u5f0f\u5217\u51fa\u5fae\u536b\u661f\u7684\u7c7b\u578b\u548c\u4f4d\u70b9\uff1b\r\n\"<FASTfile>.statistics\" \uff1a\u7edf\u8ba1\u5fae\u536b\u661f\u7684\u7c7b\u578b\u548c\u9891\u6570\u3002<\/pre>\n
\u5728MISA\u7684\u4e0b\u8f7d\u9875\u9762\u4e2d\uff0c\u63d0\u4f9b\u4e863\u4e2a\u9644\u52a0\u7684 perl \u811a\u672c\uff0c\u5206\u522b\u662f\uff1aGet_est_trimmer.pl<\/a>\uff0cp3_in.pl<\/a> \u548c p3_out.pl<\/a>\u3002<\/p>\n
\u7531\u4e8eMISA\u7a0b\u5e8f\u8bfb\u53d6fasta\u6587\u4ef6\u4e2d\u7684\u5e8f\u5217ID\uff0c\u5c06\u5e8f\u5217ID\u4e2d\u7684\u7a7a\u683c\u7528\u4e0b\u5212\u7ebf \u201d_\u201d \u586b\u8865\u4e86\uff0c\u6240\u4ee5\u5728fasta\u6587\u4ef6\u4e2d\uff0c\u5176\u5e8f\u5217ID\u6700\u597d\u4e0d\u8981\u6709\u7a7a\u683c\u3002\u5426\u5219\u8fd0\u884c\u63a5\u4e0b\u6765\u7684\u7a0b\u5e8f\u65f6\uff0c\u4f1a\u51fa\u95ee\u9898\u3002<\/p>\n
Get_est_trimmer.pl<\/h1>\n
\u9488\u5bf9EST\u5e8f\u5217\uff0c\u53ef\u4ee5\u9664\u53bbEST\u5e8f\u5217\u4e2d\u77ed\u7684\u5e8f\u5217\u548c\u4e24\u7aef\u4e0d\u660e\u786e\u7684\u78b1\u57fa\u3002<\/p>\n
p3_in.pl<\/h1>\n
\u8f93\u5165 misa.pl \u7684\u8f93\u51fa\u7ed3\u679c\uff0c\u5c06\u5f15\u7269\u8bbe\u8ba1\u7684\u53c2\u6570\u6587\u4ef6\uff08\u6a21\u677f\uff0c\u4ea7\u7269\u957f\u5ea6\uff0c\u76ee\u6807\u533a\u57df\u7b49\uff09\u5bfc\u5165\u5230\u4e00\u4e2a\u4ee5\u201cp3in\u201d\u4e3a\u540e\u7f00\u7684\u6587\u4ef6\u4e2d\u3002<\/p>\n
$ p3_in.pl filename.misa<\/pre>\n
\u00a0\u8c03\u7528 primer3_core<\/h1>\n
\u8be5\u8f6f\u4ef6\u8be6\u7ec6\u89e3\u8bf4\u89c1\uff1ahttp:\/\/www.hzaumycology.com\/chenlianfu_blog\/?p=284<\/a>\uff0c\u751f\u6210\u7ed3\u679c\u6587\u4ef6 filename.p3in\u3002\u4f7f\u7528primer3-2.3.5\u7248\u672c\u7684\u65f6\u5019\uff0cMISA\u5b98\u7f51\u63d0\u4f9b\u7684p3_in.pl\u7684\u7ed3\u679c\u4e0d\u7b26\u5408primer3-2.3.5\u7684\u8f93\u5165\u683c\u5f0f\uff0c\u6545\u9700\u8981\u4fee\u6539p3_in.pl\u548cp3_out.pl\u6587\u4ef6\u3002<\/p>\n
\u00a0$ primer3_core -default_version=1 -output=filename.p3out filename.p3in<\/pre>\n
\u00a0p3_out.pl<\/h1>\n
\u5bf9primer3\u4ea7\u751f\u7684\u6587\u4ef6\u8fdb\u884c\u63d0\u53d6\u5408\uff0c\u5f97\u5230\u6700\u540e\u7684\u7ed3\u679c\u6587\u4ef6 filename.result<\/p>\n
$ p3_out.pl filename.p3out filename.misa<\/pre>\n
p3_in.pl \u548c p3_out.pl \u8fd9\u4e24\u652f\u7a0b\u5e8f\u9700\u8981\u4fee\u6539\u624d\u80fd\u6b63\u5e38\u4f7f\u7528\u3002<\/h1>\n
\u4fee\u6539\u8fc7\u540e\u7684\u4e24\u652f\u7a0b\u5e8f\u4e0b\u8f7d\uff1ap3_in.pl<\/a> \u548c p3_out.pl<\/a>\u3002<\/p>\n
\u7ed3\u679c\u6587\u4ef6\u793a\u4f8b<\/h1>\n
ID\u00a0\u00a0\u00a0\u00a0\u00a0 SSR nr. SSR type\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 SSR\u00a0\u00a0\u00a0\u00a0 size\u00a0\u00a0\u00a0 start\u00a0\u00a0 end\u00a0\u00a0\u00a0\u00a0 FORWARD PRIMER1 (5'-3') Tm(\u00b0C)\u00a0 size\u00a0\u00a0\u00a0 REVERSE PRIMER1 (5'-3') Tm(\u00b0C)\u00a0 size\u00a0\u00a0\u00a0 PRODUCT1 size (bp)\u00a0\u00a0\u00a0\u00a0\u00a0 start (bp)\u00a0\u00a0\u00a0\u00a0\u00a0 end (bp)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 FORWARD PRIMER2 (5'-3') Tm(\u00b0C)\u00a0 size\u00a0\u00a0\u00a0 REVERSE PRIMER2 (5'-3') Tm(\u00b0C)\u00a0 size\u00a0\u00a0\u00a0 PRODUCT2 size (bp)\u00a0\u00a0\u00a0\u00a0\u00a0 start (bp)\u00a0\u00a0\u00a0\u00a0\u00a0 end (bp)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 FORWARD PRIMER3 (5'-3') Tm(\u00b0C)\u00a0 size\u00a0\u00a0\u00a0 REVERSE PRIMER3 (5'-3') Tm(\u00b0C)\u00a0 size\u00a0\u00a0\u00a0 PRODUCT3 size (bp)\u00a0\u00a0\u00a0\u00a0\u00a0 start (bp)\u00a0\u00a0\u00a0\u00a0\u00a0 end (bp)\r\nscaffold1_254817_bp\u00a0\u00a0\u00a0\u00a0 1\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p3\u00a0\u00a0\u00a0\u00a0\u00a0 (GCC)5\u00a0 15\u00a0\u00a0\u00a0\u00a0\u00a0 17114\u00a0\u00a0 17128\u00a0\u00a0 TGATGTCCTAGTGCGTCTCG\u00a0\u00a0\u00a0 60.008\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CATCCTGTCTTTGAACGGGT\u00a0\u00a0\u00a0 59.966\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 226\u00a0\u00a0\u00a0\u00a0 17022\u00a0\u00a0 17247\u00a0\u00a0 TGATGTCCTAGTGCGTCTCG\u00a0\u00a0\u00a0 60.008\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 ACATCCTGTCTTTGAACGGG\u00a0\u00a0\u00a0 59.966\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 227\u00a0\u00a0\u00a0\u00a0 17022\u00a0\u00a0 17248\u00a0\u00a0 TGATGTCCTAGTGCGTCTCG\u00a0\u00a0\u00a0 60.008\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 TGAGGGAGTTGTGGTGATGA\u00a0\u00a0\u00a0 60.088\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 142\u00a0\u00a0\u00a0\u00a0 17022\u00a0\u00a0 17163\r\nscaffold1_254817_bp\u00a0\u00a0\u00a0\u00a0 2\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p1\u00a0\u00a0\u00a0\u00a0\u00a0 (T)10\u00a0\u00a0 10\u00a0\u00a0\u00a0\u00a0\u00a0 116694\u00a0 116703\u00a0 ATTGCAACCACCAAAGAAGG\u00a0\u00a0\u00a0 59.971\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CTCCAGGCGCTACGTTAATC\u00a0\u00a0\u00a0 59.867\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 151\u00a0\u00a0\u00a0\u00a0 116600\u00a0 116750\u00a0 TCCCTACTGCATTGACCTCC\u00a0\u00a0\u00a0 60.073\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CTCCAGGCGCTACGTTAATC\u00a0\u00a0\u00a0 59.867\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 223\u00a0\u00a0\u00a0\u00a0 116528\u00a0 116750\u00a0 ACACTGCCTTCGATTCATCC\u00a0\u00a0\u00a0 60.081\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CTCCAGGCGCTACGTTAATC\u00a0\u00a0\u00a0 59.867\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 246\u00a0\u00a0\u00a0\u00a0 116505\u00a0 116750\r\nscaffold1_254817_bp\u00a0\u00a0\u00a0\u00a0 3\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p3\u00a0\u00a0\u00a0\u00a0\u00a0 (TTG)5\u00a0 15\u00a0\u00a0\u00a0\u00a0\u00a0 142162\u00a0 142176\u00a0 TCCACAACCCAATTTACGGT\u00a0\u00a0\u00a0 60.088\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CCAGAGTATGCCTGGTTCGT\u00a0\u00a0\u00a0 60.134\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 212\u00a0\u00a0\u00a0\u00a0 142004\u00a0 142215\u00a0 GTCCACAACCCAATTTACGG\u00a0\u00a0\u00a0 60.088\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CCAGAGTATGCCTGGTTCGT\u00a0\u00a0\u00a0 60.134\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 213\u00a0\u00a0\u00a0\u00a0 142003\u00a0 142215\u00a0 GCCAGTTTTGACAGGCGTAT\u00a0\u00a0\u00a0 60.140\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CCAGAGTATGCCTGGTTCGT\u00a0\u00a0\u00a0 60.134\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 236\u00a0\u00a0\u00a0\u00a0 141980\u00a0 142215\r\nscaffold1_254817_bp\u00a0\u00a0\u00a0\u00a0 4\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p3\u00a0\u00a0\u00a0\u00a0\u00a0 (AGG)5\u00a0 15\u00a0\u00a0\u00a0\u00a0\u00a0 145861\u00a0 145875\u00a0 TTCGAGCTCGTCTGGTAGGT\u00a0\u00a0\u00a0 60.012\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 ATTTATCGTCCAGTGCCCAG\u00a0\u00a0\u00a0 59.955\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 212\u00a0\u00a0\u00a0\u00a0 145725\u00a0 145936\u00a0 GTTCGAGCTCGTCTGGTAGG\u00a0\u00a0\u00a0 60.012\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 ATTTATCGTCCAGTGCCCAG\u00a0\u00a0\u00a0 59.955\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 213\u00a0\u00a0\u00a0\u00a0 145724\u00a0 145936\u00a0 GGTTCGAGCTCGTCTGGTAG\u00a0\u00a0\u00a0 60.012\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 ATTTATCGTCCAGTGCCCAG\u00a0\u00a0\u00a0 59.955\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 214\u00a0\u00a0\u00a0\u00a0 145723\u00a0 145936\r\nscaffold1_254817_bp\u00a0\u00a0\u00a0\u00a0 5\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p3\u00a0\u00a0\u00a0\u00a0\u00a0 (CAT)6\u00a0 18\u00a0\u00a0\u00a0\u00a0\u00a0 182964\u00a0 182981\u00a0 TGTAGAGGGAGGCTGAGGAA\u00a0\u00a0\u00a0 59.943\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 TTGCGAAAAGCAAGGAGAGT\u00a0\u00a0\u00a0 60.132\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 270\u00a0\u00a0\u00a0\u00a0 182913\u00a0 183182\u00a0 TGTAGAGGGAGGCTGAGGAA\u00a0\u00a0\u00a0 59.943\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 GCAAGGAGAGTCGGGTATGA\u00a0\u00a0\u00a0 60.218\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 261\u00a0\u00a0\u00a0\u00a0 182913\u00a0 183173\u00a0 TGTAGAGGGAGGCTGAGGAA\u00a0\u00a0\u00a0 59.943\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 AAAAGCAAGGAGAGTCGGGT\u00a0\u00a0\u00a0 60.247\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 265\u00a0\u00a0\u00a0\u00a0 182913\u00a0 183177\r\nscaffold1_254817_bp\u00a0\u00a0\u00a0\u00a0 6\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p3\u00a0\u00a0\u00a0\u00a0\u00a0 (GAT)5\u00a0 15\u00a0\u00a0\u00a0\u00a0\u00a0 220964\u00a0 220978\u00a0 ATTGATACCGGTGGGTGAAA\u00a0\u00a0\u00a0 60.051\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 TTGAAGGAACTTCGAATGGG\u00a0\u00a0\u00a0 60.044\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 263\u00a0\u00a0\u00a0\u00a0 220929\u00a0 221191\u00a0 ATTGATACCGGTGGGTGAAA\u00a0\u00a0\u00a0 60.051\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 TCGAATGGGATCAACTTTCC\u00a0\u00a0\u00a0 59.871\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 252\u00a0\u00a0\u00a0\u00a0 220929\u00a0 221180\u00a0 ATTGATACCGGTGGGTGAAA\u00a0\u00a0\u00a0 60.051\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 GGAACTTCGAATGGGATCAA\u00a0\u00a0\u00a0 59.871\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 258\u00a0\u00a0\u00a0\u00a0 220929\u00a0 221186\r\nscaffold1_254817_bp\u00a0\u00a0\u00a0\u00a0 7\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p2\u00a0\u00a0\u00a0\u00a0\u00a0 (TA)7\u00a0\u00a0 14\u00a0\u00a0\u00a0\u00a0\u00a0 249504\u00a0 249517\u00a0 TACCATGAGAAGGGGGAATG\u00a0\u00a0\u00a0 59.744\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 TTTTCTCGACACGTCTGCAC\u00a0\u00a0\u00a0 60.032\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 230\u00a0\u00a0\u00a0\u00a0 249469\u00a0 249698\u00a0 AGAAGGGGGAATGCAAAGTC\u00a0\u00a0\u00a0 60.443\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 TTTTCTCGACACGTCTGCAC\u00a0\u00a0\u00a0 60.032\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 223\u00a0\u00a0\u00a0\u00a0 249476\u00a0 249698\u00a0 GAGAAGGGGGAATGCAAAGT\u00a0\u00a0\u00a0 60.443\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 TTTTCTCGACACGTCTGCAC\u00a0\u00a0\u00a0 60.032\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 224\u00a0\u00a0\u00a0\u00a0 249475\u00a0 249698\r\nscaffold2_167145_bp\u00a0\u00a0\u00a0\u00a0 1\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p3\u00a0\u00a0\u00a0\u00a0\u00a0 (CTG)7\u00a0 21\u00a0\u00a0\u00a0\u00a0\u00a0 3622\u00a0\u00a0\u00a0 3642\u00a0\u00a0\u00a0 GGAGATATTTCCTCAGGGGC\u00a0\u00a0\u00a0 59.866\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 AGGCAATGTCGATGCTATCC\u00a0\u00a0\u00a0 60.066\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 240\u00a0\u00a0\u00a0\u00a0 3445\u00a0\u00a0\u00a0 3684\u00a0\u00a0\u00a0 AGAAGCAGAAGGAGGTGCAG\u00a0\u00a0\u00a0 59.745\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 AGGCAATGTCGATGCTATCC\u00a0\u00a0\u00a0 60.066\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 184\u00a0\u00a0\u00a0\u00a0 3501\u00a0\u00a0\u00a0 3684\u00a0\u00a0\u00a0 GGGAGATATTTCCTCAGGGG\u00a0\u00a0\u00a0 59.722\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 AGGCAATGTCGATGCTATCC\u00a0\u00a0\u00a0 60.066\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 241\u00a0\u00a0\u00a0\u00a0 3444\u00a0\u00a0\u00a0 3684\r\nscaffold2_167145_bp\u00a0\u00a0\u00a0\u00a0 2\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p2\u00a0\u00a0\u00a0\u00a0\u00a0 (AT)6\u00a0\u00a0 12\u00a0\u00a0\u00a0\u00a0\u00a0 82759\u00a0\u00a0 82770\u00a0\u00a0 CCATCCCTCTTCCTCTTCCT\u00a0\u00a0\u00a0 59.630\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 ACAAGGTGATGCACAATCCA\u00a0\u00a0\u00a0 59.967\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 222\u00a0\u00a0\u00a0\u00a0 82648\u00a0\u00a0 82869\u00a0\u00a0 CCATCCCTCTTCCTCTTCCT\u00a0\u00a0\u00a0 59.630\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CACAAGGTGATGCACAATCC\u00a0\u00a0\u00a0 59.967\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 223\u00a0\u00a0\u00a0\u00a0 82648\u00a0\u00a0 82870\u00a0\u00a0 CCATCCCTCTTCCTCTTCCT\u00a0\u00a0\u00a0 59.630\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 CCACAAGGTGATGCACAATC\u00a0\u00a0\u00a0 59.967\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 224\u00a0\u00a0\u00a0\u00a0 82648\u00a0\u00a0 82871\r\nscaffold3_156598_bp\u00a0\u00a0\u00a0\u00a0 1\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 c\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 (GGA)5(GGT)5\u00a0\u00a0\u00a0 30\u00a0\u00a0\u00a0\u00a0\u00a0 70534\u00a0\u00a0 70563\u00a0\u00a0 GGTGGATGTATTGGATTGCC\u00a0\u00a0\u00a0 60.021\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 GGCATGAACGACTTTTTGCT\u00a0\u00a0\u00a0 60.257\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 183\u00a0\u00a0\u00a0\u00a0 70469\u00a0\u00a0 70651\u00a0\u00a0 GGGTGGATGTATTGGATTGC\u00a0\u00a0\u00a0 60.021\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 GGCATGAACGACTTTTTGCT\u00a0\u00a0\u00a0 60.257\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 184\u00a0\u00a0\u00a0\u00a0 70468\u00a0\u00a0 70651\u00a0\u00a0 TGGTCATCGAGCTGATGGTA\u00a0\u00a0\u00a0 60.225\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 GGCATGAACGACTTTTTGCT\u00a0\u00a0\u00a0 60.257\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 229\u00a0\u00a0\u00a0\u00a0 70423\u00a0\u00a0 70651\r\nscaffold3_156598_bp\u00a0\u00a0\u00a0\u00a0 2\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 p3\u00a0\u00a0\u00a0\u00a0\u00a0 (CGC)6\u00a0 18\u00a0\u00a0\u00a0\u00a0\u00a0 80301\u00a0\u00a0 80318\u00a0\u00a0 CCGAAAAGGCCATTAGTTCA\u00a0\u00a0\u00a0 60.067\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 ACGACGAATGAAACCCTTTG\u00a0\u00a0\u00a0 59.971\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 255\u00a0\u00a0\u00a0\u00a0 80206\u00a0\u00a0 80460\u00a0\u00a0 CCGAAAAGGCCATTAGTTCA\u00a0\u00a0\u00a0 60.067\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 TTGGGGTGAGTTCCTTATCG\u00a0\u00a0\u00a0 59.926\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 238\u00a0\u00a0\u00a0\u00a0 80206\u00a0\u00a0 80443\u00a0\u00a0 TCTTTTGACTTCGATGCCCT\u00a0\u00a0\u00a0 59.813\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 ACGACGAATGAAACCCTTTG\u00a0\u00a0\u00a0 59.971\u00a0 20\u00a0\u00a0\u00a0\u00a0\u00a0 216\u00a0\u00a0\u00a0\u00a0 80245\u00a0\u00a0 80460<\/pre>\n","protected":false},"excerpt":{"rendered":"
MISA\uff0c\u82f1\u6587\u5168\u79f0\u4e3aMIcroSAtellite identification  … \u7ee7\u7eed\u9605\u8bfb →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[22],"_links":{"self":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/255"}],"collection":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=255"}],"version-history":[{"count":16,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/255\/revisions"}],"predecessor-version":[{"id":1946,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/255\/revisions\/1946"}],"wp:attachment":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=255"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=255"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=255"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}