{"id":3835,"date":"2024-03-25T18:24:22","date_gmt":"2024-03-25T10:24:22","guid":{"rendered":"http:\/\/www.chenlianfu.com\/?p=3835"},"modified":"2024-03-26T00:14:27","modified_gmt":"2024-03-25T16:14:27","slug":"%e5%b8%b8%e7%94%a8%e7%9a%84%e7%89%a9%e7%a7%8d%e5%88%86%e7%b1%bb%e9%89%b4%e5%ae%9a%e5%9f%ba%e5%9b%a0","status":"publish","type":"post","link":"http:\/\/www.chenlianfu.com\/?p=3835","title":{"rendered":"\u5e38\u7528\u7684\u7269\u79cd\u5206\u7c7b\u9274\u5b9a\u57fa\u56e0\u6216\u5e8f\u5217"},"content":{"rendered":"\n

LSU rDNA\uff0clarge subunit rDNA\uff0c\u6838\u7cd6\u4f53\u5927\u4e9a\u57fa\u8131\u6c27\u6838\u7cd6\u6838\u9178\u3002LSU rDNA\u5e8f\u5217\u80fd\u8f6c\u5f55\u51faLSU rRNA\uff08\u6838\u7cd6\u4f53\u5927\u4e9a\u57fa\u6838\u7cd6\u6838\u9178\uff09\u3002LSU rRNA\u548cSSU rRNA\u662f\u6838\u7cd6\u4f53\u7684\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3002LSU rDNA\u5e8f\u5217\u957f\u5ea6\uff1a\u771f\u6838\u751f\u7269\u5728Rfam\u6570\u636e\u5e93\u7f16\u53f7\u4e3aRF02543\uff0c\u957f\u5ea63401 bp\uff1b\u7ec6\u83cc\u5728Rfam\u6570\u636e\u5e93\u7f16\u53f7RF02541\uff0c\u957f\u5ea62925 bp\uff1b\u53e4\u83cc\u5728Rfam\u6570\u636e\u5e93\u7f16\u53f7RF02540\uff0c\u957f\u5ea62990 bp\u3002\u4f7f\u7528Infernal\u8f6f\u4ef6\u5c06DNA\u5e8f\u5217\u548cRfam\u6570\u636e\u5e93\u4e2d\u7684LSU rRNA\u5bb6\u65cfcm\u6a21\u578b\u8fdb\u884c\u6bd4\u8f83\u8fdb\u884c\u9274\u5b9a\u3002<\/p>\n\n\n\n

SSU rDNA\uff0csmall subunit rDNA\uff0c\u6838\u7cd6\u4f53\u5c0f\u4e9a\u57fa\u8131\u6c27\u6838\u7cd6\u6838\u9178\u3002SSU rDNA\u5e8f\u5217\u80fd\u8f6c\u5f55\u51faSSU rRNA\uff08\u6838\u7cd6\u4f53\u5c0f\u4e9a\u57fa\u6838\u7cd6\u6838\u9178\uff09\u3002LSU rRNA\u548cSSU rRNA\u662f\u6838\u7cd6\u4f53\u7684\u4e3b\u8981\u7ec4\u6210\u6210\u5206\u3002SSU rDNA\u5e8f\u5217\u957f\u5ea6\uff1a\u771f\u6838\u751f\u7269\u5728Rfam\u6570\u636e\u5e93\u7f16\u53f7\u4e3aRF01960\uff0c\u957f\u5ea61851 bp\uff1b\u7ec6\u83cc\u5728Rfam\u6570\u636e\u5e93\u7f16\u53f7RF00177\uff0c\u957f\u5ea61533 bp\uff1b\u53e4\u83cc\u5728Rfam\u6570\u636e\u5e93\u7f16\u53f7RF01959\uff0c\u957f\u5ea61477 bp\u3002\u4f7f\u7528Infernal\u8f6f\u4ef6\u5c06DNA\u5e8f\u5217\u548cRfam\u6570\u636e\u5e93\u4e2d\u7684LSU rRNA\u5bb6\u65cfcm\u6a21\u578b\u8fdb\u884c\u6bd4\u8f83\u8fdb\u884c\u9274\u5b9a\u3002yi<\/p>\n\n\n\n

ITS region\uff0cinternal transcribed spacer region\uff0c\u5185\u8f6c\u5f55\u95f4\u9694\u533a\u3002rDNA\u5e8f\u5217\u80fd\u8f6c\u5f55\u51fa\u7684\u524d\u4f53RNA\uff08pre-RNA\uff0cprecursor RNA\uff09\u5305\u542bLSU rRNA\u548cSSU rRNA\uff0c\u4ee5\u53ca\u4e24\u8005\u4e4b\u95f4\u76845s\u62165.8s rRNA\u3002\u5728\u8be5\u524d\u4f53RNA\u5e8f\u5217\u4e0a\u4e0d\u540crRNAs\u7684\u5185\u90e8\u5e76\u5c06rRNAs\u9694\u79bb\u5f00\u7684\u8f6c\u5f55\u533a\u57df\uff0c\u5373\u4e3a\u5185\u8f6c\u5f55\u95f4\u9694\u533a\u3002\u5b83\u4e00\u822c\u5305\u542b\u4e24\u4e2a\u533a\u57dfITS1\u548cITS2\u3002\u524d\u8005\u6307SSU\u5f805s\u62165.8s\u4e4b\u95f4\u7684\u533a\u57df\uff0c\u540e\u8005\u63075s\u62165.8s\u5f80LSU\u4e4b\u95f4\u7684\u533a\u57df\u3002SSU\u548cLSU rDNA\u5e8f\u5217\u4f4d\u4e8e\u57fa\u56e0\u7ec4\u4e0a\uff0c\u4e2d\u95f4\u9694\u77405s\u62165.8s rDNA\u5e8f\u5217\u3002\u5b83\u4eec\u4e00\u8d77\u4f5c\u4e3a\u4e00\u4e2a\u91cd\u590d\u5355\u5143\uff0c\u5728\u57fa\u56e0\u7ec4\u4e0a\u4e32\u8054\u91cd\u590d\u8bb8\u591a\u6b21\u3002\u82e5\u8f6c\u5f55\u8d77\u59cb\u4f4d\u70b9\u4f4d\u4e8e\u4ee5\u4e0a\u91cd\u590d\u5355\u5143\u4e0a\u6e38\u67d0\u533a\u57df\uff0c\u5219\u76f8\u5e94\u7684\u5916\u8f6c\u5f55\u95f4\u9694\u533a\u5219\u662fLSU\u548cSSU\u4e4b\u95f4\u7684\u4e00\u6bb5\u5e8f\u5217\uff0c\u4f4d\u4e8e\u524d\u4f53RNA\u4e24\u7aef\u4e0d\u5c5e\u4e8erRNA\u7684\u5e8f\u5217\uff1b\u82e5\u8f6c\u5f55\u7684\u5bf9\u8c61\u4e0d\u662f\u4e00\u4e2a\u91cd\u590d\u5355\u5143\uff0c\u800c\u662f\u6574\u4e2a\u91cd\u590d\u5e8f\u5217\u5168\u90e8\uff0c\u5219LSU\u548cSSU\u4e4b\u95f4\u7684\u5e8f\u5217\u4e5f\u5e94\u8be5\u662f\u5185\u8f6c\u5f55\u7ec4\u95f4\u9694\u533a\uff0c\u6216\u8005\u79f0\u4e3aITS3\u533a\u57df\u3002\u5728\u771f\u548c\u751f\u7269\u4e2d\uff0cSSU \u7684\u6c89\u964d\u7cfb\u6570\u662f18s\uff0cLSU\u662f28s\uff1b\u5728\u539f\u6838\u751f\u7269\u4e2dSSU\u662f16s\uff0cLSU\u662f26s\u3002\u5728\u539f\u6838\u751f\u7269\u4e2d\u4ec5\u5b58\u57285s rRNA\uff1b\u5728\u771f\u6838\u751f\u7269\u57fa\u56e0\u7ec4\u4e2d\u4e00\u822c\u540c\u65f6\u67095s\u548c5.8s rRNA\u3002\u5728\u771f\u6838\u751f\u7269\u4e2d\uff0crDNA\u91cd\u590d\u5355\u5143\u4e2d\u4e00\u822c\u4ec5\u67095.8s\uff0c\u4f46\u6709\u4e9b\u7269\u79cd\u4e5f\u5305\u542b5s\u3002\u7531\u4e8eLSU\u548cSSU rDNA\u5e8f\u5217\u975e\u5e38\u4fdd\u5b88\uff0c\u53ef\u80fd\u65e0\u6cd5\u7528\u4e8e\u7269\u79cd\u7684\u533a\u5206\u3002\u6b64\u65f6\uff0c\u91c7\u7528\u8fdb\u5316\u901f\u5ea6\u7ea6\u9ad810\u500d\u7684ITS\u5e8f\u5217\u8fdb\u884c\u9274\u5b9a\u662f\u66f4\u52a0\u6709\u6548\u7684\u3002\u82e5\u9700\u8981\u68c0\u6d4b\u57fa\u56e0\u7ec4\u4e0a\u7684ITS\u5e8f\u5217\uff0c\u5219\u9700\u8981\u5148\u4f7f\u7528Infernal\u8f6f\u4ef6\u57fa\u4e8eRfam\u6570\u636e\u9274\u5b9a\u51faLSU\u3001SSU\u30015s\u548c5.8s rDNA\u5e8f\u5217\u540e\uff0c\u5728\u5206\u6790ITS1\u548cITS2\u5e8f\u5217\u3002<\/p>\n\n\n\n

RPB2\uff0cRNA\u00a0polymerase\u00a0beta(II)\u00a0subunit\u00a02\uff0cRNA\u805a\u5408\u9176II\u7b2c\u4e8c\u4e9a\u57fa\u3002RNA\u805a\u5408\u9176II\u7528\u4e8eDNA\u4ecb\u5bfc\u4e0b\u5408\u6210mRNA\uff0c\u5728\u9057\u4f20\u4fe1\u606f\u7684\u8f6c\u5f55\u4e2d\u53d1\u6325\u91cd\u8981\u4f5c\u7528\u3002\u5b83\u753112\u4e2a\u4e9a\u57fa\u6784\u6210\uff0c\u5176\u4e2d\u6700\u5927\u7684\u4e9a\u57fa\u662fRPB1\uff0c\u7b2c\u4e8c\u5927\u7684\u4e9a\u57fa\u662fRPB2\u3002RPB1\u57fa\u56e0\u901a\u5e38\u5728\u5927\u90e8\u5206\u7269\u79cd\u4e2d\u662f\u591a\u62f7\u8d1d\u7684\uff0c\u800cRPB2\u57fa\u56e0\u5219\u662f\u5355\u62f7\u8d1d\u7684\u3002\u6545RPB2\u57fa\u56e0\u6bd4RPB1\u66f4\u9002\u5408\u4e8e\u7269\u79cd\u7684\u5206\u7c7b\u548c\u9274\u5b9a\u3002\u8be5\u86cb\u767d\u5e8f\u5217\u6bd4\u8f83\u5927\uff0c\u6709\u7ea61200 aa\uff0c\u53ef\u4f7f\u7528Pfam\u6570\u636e\u5e93\u8fdb\u884c\u9274\u5b9a\uff0c\u8981\u6c42\u5176\u86cb\u767d\u5e8f\u5217\u540c\u65f6\u5177\u67097\u4e2a\u7ed3\u6784\u57df\uff08PF04563\u3001PF04561\u3001PF04565\u3001PF04566\u3001PF04567\u3001PF00562\u3001PF04560\uff09\uff0c\u624d\u80fd\u9274\u5b9a\u5176\u4e3aRPB2\u86cb\u767d\u3002\u56e0\u4e3aRNA\u805a\u5408\u9176I\u3001II\u548cIII\u7b2c\u4e8c\u5927\u4e9a\u57fa\u7684\u86cb\u767d\u5e8f\u5217\u662f\u975e\u5e38\u76f8\u4f3c\u7684\uff0c\u6574\u4e2a\u57fa\u56e0\u7ec4\u4e2d\u53ef\u80fd\u67092~3\u4e2a\u57fa\u56e0\u7684\u86cb\u767d\u5e8f\u5217\u90fd\u80fd\u9274\u5b9a\u5230\u8fd97\u4e2a\u7ed3\u6784\u57df\uff0c\u6b64\u65f6\u9009\u62e9\u5176\u4e2d\u6700\u4f18\u7684\u57fa\u56e0\uff08\u5bf97\u4e2a\u7ed3\u6784\u57df\u7684\u8986\u76d6\u7387\u9ad8\u8fbe98~100%\uff09\u4f5c\u4e3aRPB2\u57fa\u56e0\u3002<\/p>\n\n\n\n

TUBB\uff0ctubulin beta chain\uff0c\u03b2-tubulin\u3002\u03b2-tubulin\u662f\u5fae\u7ba1\u86cb\u767d\u4e09\u79cd\u4e9a\u57fa\u4e4b\u4e00\uff0c\u7ec6\u80de\u9aa8\u67b6\u4e2d\u7684\u91cd\u8981\u7ec4\u6210\u6210\u5206\u3002\u7ec6\u80de\u9aa8\u67b6\u662f\u7ec6\u80de\u5185\u7684\u4e00\u79cd\u652f\u6301\u7ed3\u6784\uff0c\u5b83\u5305\u62ec\u5fae\u7ba1\u3001\u5fae\u4e1d\u548c\u4e2d\u95f4\u4e1d\u7b49\u7ed3\u6784\uff0c\u8d77\u5230\u7ef4\u6301\u7ec6\u80de\u5f62\u6001\u3001\u7ec6\u80de\u8fd0\u52a8\u4ee5\u53ca\u7ec6\u80de\u5185\u7269\u8d28\u8fd0\u8f93\u7684\u91cd\u8981\u4f5c\u7528\u3002\u5fae\u7ba1\u86cb\u767d\u662f\u7531\u03b1-\u3001\u03b2-\u5fae\u7ba1\u86cb\u767d\u5206\u5b50\u805a\u5408\u800c\u6210\u7684\u5f02\u4e8c\u805a\u4f53\uff0c\u03b1-\u548c\u03b2-\u4e24\u79cd\u4e9a\u57fa\u5206\u522b\u4e0eGTP\u548cGDP\u7ed3\u5408\u3002\u03b2-\u5fae\u7ba1\u86cb\u767d\u662f\u5fae\u7ba1\u7684\u52a8\u6001\u5355\u4f4d\uff0c\u5176\u7ec4\u88c5\u540e\u5373\u5728 \u03b2-\u5fae\u7ba1\u86cb\u767d\u5904\u53d1\u751f GTP \u6c34\u89e3\u3002\u5c06\u86cb\u767d\u5e8f\u5217\u548cSwiss-Prot\u6570\u636e\u5e93\u8fdb\u884c\u6bd4\u5bf9\uff0c\u5176\u7ed3\u679c\u5e94\u8be5\u662f\u201cTubulin beta chain\u201d\u3002\u4e0d\u63a8\u8350\u4f7f\u7528Pfam\u6570\u636e\u5e93\u5bf9\u03b2-tubulin\u86cb\u767d\u8fdb\u884c\u9274\u5b9a\uff0c\u56e0\u4e3aalpha\u3001beta\u548cgamma\u4e09\u79cd\u7c7b\u578b\u7684tubulin chains\u90fd\u5177\u6709PF00091\uff08Tubulin\/FtsZ family, GTPase domain\uff09\u548cPF03953\uff08Tubulin C-terminal domain\uff09\u7ed3\u6784\u57df\u3002\u03b2-tubulin\u57fa\u56e0\u4e00\u822c\u662f\u591a\u62f7\u8d1d\u57fa\u56e0\u3002<\/p>\n\n\n\n

EF-Tu\uff0cEF-1A\uff0cEF-1alpha\uff0celongation factor Tu\uff0c\u5ef6\u4f38\u56e0\u5b50Tu\u3002EF-Tu\u5728\u86cb\u767d\u8d28\u5408\u6210\u8fc7\u7a0b\u4e2d\u7684\u4f5c\u7528\u662f\u786e\u4fdd\u6b63\u786e\u7684\u6c28\u9170-tRNA\u9009\u62e9\uff0c\u5e76\u4fc3\u8fdb\u86cb\u767d\u8d28\u94fe\u7684\u5ef6\u957f\u3002\u5b83\u80fd\u591f\u4e0e\u6c28\u9170-tRNA\u5f62\u6210\u4e00\u4e2a\u7a33\u5b9a\u7684\u590d\u5408\u7269\uff0c\u5c06\u5176\u5e26\u5230\u6838\u7cd6\u4f53A\u4f4d\u4e0a\uff0c\u4f7f\u6c28\u9170-tRNA\u4e0emRNA\u4e0a\u7684\u5bc6\u7801\u5b50\u5b8c\u5168\u914d\u5bf9\u3002\u7136\u540e\uff0c\u6838\u7cd6\u4f53\u901a\u8fc7\u6c34\u89e3\u4e09\u78f7\u9178\u817a\u82f7\uff08GTP\uff09\u6765\u91ca\u653eEF-Tu\uff0c\u5e76\u7ee7\u7eed\u86cb\u767d\u8d28\u7684\u5408\u6210\u3002EF-Tu\u5305\u542b3\u4e2a\u7ed3\u6784\u57dfPF00009\uff08Elongation factor Tu GTP binding domain\uff09\u3001PF03144\uff08Elongation factor Tu domain 2\uff09 \u548cPF03143\uff08Elongation factor Tu C-terminal domain\uff09\u3002\u53ef\u4f7f\u7528Pfam\u6570\u636e\u5e93\u8fdb\u884c\u5206\u6790\uff0c\u8981\u6c42\u5176\u86cb\u767d\u5e8f\u5217\u540c\u65f6\u5177\u6709\u4ee5\u4e0a3\u4e2a\u7ed3\u6784\u57df\uff0c\u5219\u9274\u5b9a\u4e3aEF-Tu\u57fa\u56e0\u3002EF-Tu\u57fa\u56e0\u4e00\u822c\u662f\u591a\u62f7\u8d1d\u7684\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

LSU rDNA\uff0clarge subunit rD … \u7ee7\u7eed\u9605\u8bfb →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/3835"}],"collection":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3835"}],"version-history":[{"count":4,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/3835\/revisions"}],"predecessor-version":[{"id":3840,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/3835\/revisions\/3840"}],"wp:attachment":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3835"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3835"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3835"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}