{"id":578,"date":"2013-02-18T10:32:05","date_gmt":"2013-02-18T02:32:05","guid":{"rendered":"http:\/\/www.hzaumycology.com\/chenlianfu_blog\/?p=578"},"modified":"2013-02-18T11:02:11","modified_gmt":"2013-02-18T03:02:11","slug":"%e4%b8%8a%e7%8f%ad%e7%ac%ac%e4%b8%80%e5%a4%a9%e7%9a%84%e8%a7%84%e5%88%92","status":"publish","type":"post","link":"http:\/\/www.chenlianfu.com\/?p=578","title":{"rendered":"\u4e0a\u73ed\u7b2c\u4e00\u5929\u7684\u89c4\u5212"},"content":{"rendered":"<p>\u76ee\u6807\uff1a<br \/>\n1. \u5c06\u6587\u7ae0\u6574\u51fa\u6765\uff0c\u4e89\u53d6\u57286\u6708\u4efd\u6bd5\u4e1a\uff0c\u6216\u572812\u6708\u6bd5\u4e1a\u3002\u4e0d\u80fd\u53bb\u62d6\u4e86\u3002<br \/>\n2. \u5c06\u7231\u60c5\u575a\u6301\u5230\u5e95\u3002<\/p>\n<p>\u4e00\uff1a\u6bd5\u4e1a\u4e8b\u60c5<br \/>\n\u6587\u7ae01\uff1a\u9605\u8bfb\u6587\u732e\uff0c\u770b\u770b\u53d1\u8868\u7684\u771f\u83cc\u57fa\u56e0\u7ec4\u4e2d\u90a3\u4e9b\u6709bac\u6d4b\u5e8f\u548cillumina\u6d4b\u5e8f\u540c\u65f6\u8fdb\u884c\u7684\uff0c\u7136\u540e\u4f7f\u7528\u4e0d\u540c\u7684denovo\u57fa\u56e0\u7ec4\u7ec4\u88c5\u8f6f\u4ef6\u5bf9short reads\u8fdb\u884c\u7ec4\u88c5\uff0c\u548cbac\u6d4b\u5e8f\u7684\u7ed3\u679c\u8fdb\u884c\u6bd4\u8f83\uff0c\u7136\u540e\u8bc4\u4f30\u5404\u4e2a\u7ec4\u88c5\u8f6f\u4ef6\u5bf9\u771f\u83cc\u57fa\u56e0\u7ec4\u7684\u7ec4\u88c5\u6548\u679c\u3002 \u4eae\u70b9\uff1a<1>\u9996\u6b21\u8bc4\u4f30\u5404\u4e2adenovo\u8f6f\u4ef6\u4e13\u95e8\u9488\u5bf9\u771f\u83cc\u57fa\u56e0\u7ec4\u7684\u7ec4\u88c5\u6548\u679c;<2>soapdenovo2\u53d1\u5e03\uff0c\u9996\u6b21\u5c06\u6b64\u8f6f\u4ef6\u52a0\u5165\u8bc4\u4f30\u3002 \u6587\u732e\u67e5\u9605\u4e0e\u8d44\u6599\u4e0b\u8f7d\u9700\u8981\u4e00\u4e2a\u6708\uff0c\u73b0\u5728\u52303\u6708\u4e2d\u65ec\uff1b\u57fa\u56e0\u7ec4\u7ec4\u88c5\u8f6f\u4ef6\u5b66\u4e60\u4e0e\u6570\u636e\u5206\u6790\u9700\u89812\u4e2a\u6708\uff0c\u52305\u6708\u4e2d\u65ec\uff1b\u6587\u7ae0\u64b0\u5199\u9700\u89811\u4e2a\u6708\uff0c\u52306\u6708\u4e2d\u65ec\uff1b\u6587\u7ae0\u6295\u9012\u5230\u63a5\u6536\uff0c\u9700\u89812\u4e2a\u6708\uff0c\u52308\u6708\u4efd\u3002<br \/>\n\u6587\u7ae02\uff1a\u9ed1\u6728\u8033\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5df2\u7ecf\u5206\u6790\u4e86\u4e00\u534a\u3002\u5728\u7ed3\u5408KEGG\u4ee3\u8c22\u9014\u5f84\uff0c\u6765\u8fdb\u884c\u4e00\u4e9b\u5206\u6790\uff0c\u53ef\u4ee5\u5199\u51fa\u6587\u7ae0\u3002\u5206\u6790\u9700\u89812\u4e2a\u6708\uff0c\u6587\u732e\u9605\u8bfb\u548c\u6587\u7ae0\u64b0\u5199\u9700\u89812\u4e2a\u6708\u3002<br \/>\n\u6587\u7ae03\uff1a\u9999\u83c7\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5e76\u5206\u6790\uff0c\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u73b0\u5728\u6b63\u5728\u8fdb\u884c\u4e2d\uff0c\u4f30\u8ba1300kb\u6587\u5e93\u7684\u6d4b\u5e8f\u7ed3\u679c\u5df2\u7ecf\u51fa\u6765\uff0c\u53ef\u4ee5\u5bf9\u57fa\u56e0\u7ec4\u8fdb\u884c\u8bc4\u4f30\u4e86\u3002\u7136\u540e3\u6708\u4efd\u5f00\u59cb\u591a\u4e2a\u6587\u5e93\u7684\u6d4b\u5e8f\uff0c5\u6708\u4efd\u524d\u80fd\u62ff\u5230\u6570\u636e\u548c\u7ec4\u88c5\u7ed3\u679c\u30026\uff5e8\u6708\u4efd\u80fd\u5c06\u9999\u83c7\u57fa\u56e0\u7ec4\u5206\u6790\u5b8c\u6bd5\uff0c9\u6708\u4efd\u64b0\u5199\u9999\u83c7\u57fa\u56e0\u7ec4\u6587\u7ae0\u3002\u53ef\u4ee5\u5c06\u6587\u7ae0\u53d1\u9001\u7ed9\u540d\u6c14\u4e0d\u9519\u7684\u8001\u5916\u4fee\u6539\uff0c\u7136\u540e\u8054\u5408\u53d1\u8868\u3002\u76ee\u6807\u6742\u5fd7plos one \u6216 BMC genomics\u3002<\/p>\n<p>\u4e2d\u9014\u8fd8\u4f1a\u6709\u5176\u5b83\u4e8b\u60c5\uff0c\u6bd4\u5982\u5f90\u8001\u5e08\u7684\u832f\u82d3\u8f6c\u5f55\u7ec4\u548cDGE\u5206\u6790\uff0c\u5468\u8001\u5e08\u7684\u6570\u636e\u4e0a\u4f20\uff0c\u548c\u8881\u5ca9\u7684\u86cb\u767d\u7684\u53ef\u53d8\u526a\u5207\u7b49\u3002<\/p>\n<p>\u4e8c\uff1a\u7231\u60c5<br \/>\n1. \u5e73\u65f6\u4e0d\u80fd\u5728\u65e0\u804a\u5730\u95f2\u901b\u4e0a\u7f51\u7b49\uff0c\u5728\u5de5\u4f5c\u65f6\u95f4\u4e4b\u5916\uff0c\u5927\u90e8\u5206\u65f6\u95f4\u90fd\u7528\u6765\u966a\u9093\u857e\uff1b<br \/>\n2. \u6309\u9093\u857e\u7684\u610f\u613f\uff0c\u53bb\u901b\u901b\u6b66\u6c49\u98ce\u666f\uff1b<br \/>\n3. \u6709\u4e0d\u9519\u7684\u7535\u5f71\u5f00\u5e55\uff0c\u5c31\u966a\u53bb\u770b\u7535\u5f71\uff1b<br \/>\n4. \u5173\u6ce8\u9093\u857e\u7684\u627e\u5de5\u4f5c\u4e8b\u5b9c\uff1b<br \/>\n5. \u5173\u6ce8\u9093\u857e\u5b9e\u9a8c\u95ee\u9898\u5e76\u5168\u529b\u89e3\u51b3\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u76ee\u6807\uff1a 1. \u5c06\u6587\u7ae0\u6574\u51fa\u6765\uff0c\u4e89\u53d6\u57286\u6708\u4efd\u6bd5\u4e1a\uff0c\u6216\u572812\u6708\u6bd5\u4e1a\u3002\u4e0d\u80fd\u53bb\u62d6\u4e86\u3002 2. &hellip; <a href=\"http:\/\/www.chenlianfu.com\/?p=578\">\u7ee7\u7eed\u9605\u8bfb <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[4],"tags":[],"_links":{"self":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/578"}],"collection":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=578"}],"version-history":[{"count":3,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/578\/revisions"}],"predecessor-version":[{"id":580,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=\/wp\/v2\/posts\/578\/revisions\/580"}],"wp:attachment":[{"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=578"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=578"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.chenlianfu.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=578"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}